Last data update: May 13, 2024. (Total: 46773 publications since 2009)
Records 1-10 (of 10 Records) |
Query Trace: Stommel M[original query] |
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miRNA extracted from extracellular vesicles is a robust biomarker of amyotrophic lateral sclerosis.
Banack SA , Dunlop RA , Stommel EW , Mehta P , Cox PA . J Neurol Sci 2022 442 120396 BACKGROUND AND OBJECTIVES: We examined miRNA biomarkers for ALS extracted from extracellular vesicles in blood samples using a large and diverse patient and control population. Different blood collection and storage protocols by different investigators could impact repeatability of miRNA analysis. We tested the hypotheses that miRNA extracted from extracellular vesicles using immunoaffinity purification techniques are robust and repeatable across investigators, laboratories and in a broad ALS population. METHODS: De-identified patient blood plasma samples obtained from the U.S. National ALS Biorepository were compared with plasma from non-ALS controls. Extracellular vesicles were extracted and isolated using L1CAM immunoaffinity purification. Total RNA was extracted, and miRNA quantified using qPCR following careful quality control measures. Gene fold expressions of eight miRNAs were compared using a Mann-Whitney two-tailed test. RESULTS: One hundred blinded, blood plasma samples were analyzed. Thirty-five men and 15 women with ALS were compared with controls consisting of 30 men and 20 women. None of the ALS patient cohort reported family members with ALS suggesting sporadic ALS. Five of the eight biomarkers previously published were found to significantly discriminate ALS patient samples from control samples. DISCUSSION: The methods used in this study provide a repeatable measure of miRNA biomarkers that statistically differentiate ALS patient samples from control samples. The broad inclusion criteria for both the ALS patient cohort and controls along with the collection of blood samples by different investigators suggest that these methods are robust and represent good candidates for further research and development aimed at clinical application. |
The Incidence of Amyotrophic Lateral Sclerosis in Ohio 2016-2018: The Ohio Population-Based ALS Registry
Andrew AS , Pioro EP , Li M , Shi X , Gui J , Stommel EW , Butt TH , Peipert D , Henegan P , Tischbein M , Cazzolli P , Novak J , Quick A , Pugar KD , Sawlani K , Katirji B , Hayes TA , Horton DK , Mehta P , Bradley WG . Neuroepidemiology 2021 55 (3) 1-10 INTRODUCTION: Amyotrophic lateral sclerosis (ALS) is a fatal, neuromuscular disease with no cure. ALS incidence rates have not been assessed specifically in Ohio, yet the state contains both metropolitan and rural areas with a variety of environmental factors that could contribute to disease etiology. We report the incidence of ALS in Ohio residents diagnosed from October 2016 through September 2018. METHODS: We engaged practitioners from 9 Ohio sites to identify newly diagnosed ALS patients and to complete case report forms with demographic and clinical information. ALS was diagnosed according to the Awaji criteria and classified as either definite, probable, or possible. We developed a method to estimate missing cases using a Poisson regression model to impute cases in counties with evidence of undercounting. RESULTS: We identified 333 newly diagnosed ALS patients residing in Ohio during the 2-year index period and found incidence rates varied in the 88 state counties. After incorporating the estimated 27% of missing cases, the corrected crude annual incidence was 1.96/100,000 person-years, and the age- and gender-standardized incidence was 1.71/100,000 person-years (standardized to the 2010 US census). DISCUSSION/CONCLUSION: The estimated Ohio incidence of ALS is overall similar to that reported in other states in the USA. This study reveals a geospatial variation in incidence within the state, and areas with higher rates warrant future investigation. |
Risk factors for amyotrophic lateral sclerosis: a regional U.S. case-control study
Andrew AS , Bradley WG , Peipert D , Butt T , Amoako K , Pioro EP , Tandan R , Novak J , Quick A , Pugar KD , Sawlani K , Katirji B , Hayes TA , Cazzolli P , Gui J , Mehta P , Horton DK , Stommel EW . Muscle Nerve 2020 63 (1) 52-59 INTRODUCTION: Most amyotrophic lateral sclerosis (ALS) cases are considered sporadic, without a known genetic basis, and environmental exposures are thought to play a causal role. METHODS: To learn more about sporadic ALS etiology, we recruited n=188 ALS patients from Northern New England and Ohio and matched controls 2:1 from the general population of the same regions. Questionnaires evaluated the association between a variety of lifestyle, behavioral (i.e., hobbies and activities), and occupational factors and the risk of ALS, including the duration of time between exposure and ALS onset, and exposure frequency. RESULTS: Head trauma was associated with increased ALS risk (adjusted OR 1.60 95% CI 1.04-2.45), with significantly greater effects for injuries occurring 10 or more years prior to symptom onset (p=0.037). ALS risk was increased for those reporting severe electrical burns (adjusted OR 2.86, 95% CI 1.37-6.03), with odds ratios highest for burns after age 30 (OR 3.14), and for burns 10 or more years prior to symptom onset (OR 3.09). Hobbies involving lead were the most strongly associated with ALS risk (adjusted OR 2.92, 95%CI 1.45-5.91). Exposures to lead 20 or more years prior to diagnosis had larger effect sizes compared to those occurring more recently. Holding a job in mechanics, painting, or construction was associated with ALS. DISCUSSION: The identification of these specific environmental factors associated with ALS highlight the need for future prospective and laboratory studies to assess causality, biological mechanisms, and find prevention or treatment opportunities. |
Keratinous biomarker of mercury exposure associated with amyotrophic lateral sclerosis risk in a nationwide U.S. study
Andrew AS , O'Brien KM , Jackson BP , Sandler DP , Kaye WE , Wagner L , Stommel EW , Horton DK , Mehta P , Weinberg CR . Amyotroph Lateral Scler Frontotemporal Degener 2020 21 1-8 Objective: The majority of cases of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS) are of unknown etiology. A proportion of these cases are likely to be attributable to contaminant exposures, although the specific environmental etiology of ALS remains largely a mystery. Certain forms of the neurotoxic metal mercury readily cross into the central nervous system. Fish is a dietary source of methylmercury, but also of beneficial components, such as omega-3 polyunsaturated fatty acids. Prior work using clinic-based studies of toenails and hair as keratinous biomarkers of exposure suggest elevated mercury levels in ALS patients compared with controls. We sought to validate this relationship in a U.S. case-control comparison of mercury levels in nail clippings. Methods: We performed trace element analysis using inductively coupled plasma mass spectrometry (ICP-MS) on the nail clippings of n = 70 female, geographically representative ALS patients from the National ALS Biorepository and compared them to n = 210 age-matched controls from a set of n = 1216 nationally distributed controls from the Sister and Two Sister Studies. Results: Compared to the lowest quartile of nail mercury, moderate levels were associated with decreased risk of ALS (P = 4.18e-6). However, the odds of having nail mercury levels above the 90th percentile were 2.3-fold higher among ALS patients compared with controls (odds ratio (OR) = 2.3, 95% confidence interval 1.10-4.58, adjusted for age and smoking status). Conclusion: This finding suggests that excessive mercury exposure may be associated with the neurodegenerative health of aging populations. |
"ALS reversals": demographics, disease characteristics, treatments, and co-morbidities
Harrison D , Mehta P , van Es MA , Stommel E , Drory VE , Nefussy B , van den Berg LH , Crayle J , Bedlack R . Amyotroph Lateral Scler Frontotemporal Degener 2018 19 1-5 OBJECTIVE: To identify differences in demographics, disease characteristics, treatments, and co-morbidities between patients with "amyotrophic lateral sclerosis (ALS) reversals" and those with typically progressive ALS. METHODS: Cases of possible ALS reversals were found in prior publications, in the Duke ALS clinic, through self-referral or referral from other Neurologists, and on the internet. Of 89 possible reversals identified, 36 cases were included because chart or literature review confirmed their diagnosis and a robust, sustained improvement in at least one objective measure. Controls were participants in the Pooled Resource Open-Access ALS Clinical Trials database and the National ALS Registry. Cases and controls were compared using descriptive statistics. RESULTS: ALS reversals were more likely to be male, have limb onset disease, and initially progress faster. The prevalences of myasthenia gravis (MG) and purely lower motor neuron disease in cases were higher than estimates of these prevalences in the general population. The odds of taking curcumin, luteolin, cannabidiol, azathioprine, copper, glutathione, vitamin D, and fish oil were greater for cases than controls. CONCLUSIONS: When compared to patients with typically progressive ALS, patients with reversals differed in their demographics, disease characteristics, and treatments. While some of these patients may have had a rare antibody-mediated ALS mimicker, such as atypical myasthenia gravis, details of their exams, EMGs and family histories argue that this was unlikely. Instead, our data suggest that ALS reversals warrant evaluation for mechanisms of disease resistance and that treatments associated with multiple ALS reversals deserve further study. |
Attempts to lose weight among US children: Importance of weight perceptions from self, parents, and health professionals
Ling J , Stommel M , Choi SH . Obesity (Silver Spring) 2018 26 (3) 597-605 OBJECTIVE: The objective of this study was to investigate how perceptions of weight by children themselves, parents, and health professionals influence children's persistent attempts to lose weight. METHODS: The sample included 4,914 children aged 8 to 15 years from the 2005 to 2014 National Health and Nutrition Examination Survey (representing 20.7 million children). Data were analyzed using logistic regression models. RESULTS: About 34.2% never made an effort to lose weight, whereas 28.2% made persistent attempts to lose weight. Children's persistent attempts to lose weight were highly related to their own BMI percentile. Children's self-perceptions of overweight increased the odds of persistent attempts to lose weight more than sevenfold. Health professionals' perceptions that children were overweight increased the odds of persistent attempts to lose weight almost threefold. However, parents' perceptions of children as overweight had a relatively small though significant influence on children's attempts to lose weight. CONCLUSIONS: As perceptions of children's weight status play big roles in children's attempts to lose weight, interventions focusing on increasing accuracy of perceptions may help promote healthy weight loss efforts. Although parents are key agents in controlling their children's weight gain, especially among minority school-aged children, the study findings also emphasize the greater importance of health professionals on children's attempts to lose weight across different racial/ethnic groups. |
Examining the high rate of cigarette smoking among adults with a GED
Schoenborn CA , Stommel M , Lucas JW . Addict Behav 2017 77 275-286 OBJECTIVES: We seek to identify characteristics of GED holders that explain their very high smoking rates compared with high school (HS) graduates. METHODS: We pooled data from the 2006-2014 National Health Interview Surveys (NHIS) for adults aged 25 and older (n=235,031) to describe cigarette smoking behaviors and smoking history for adults in six education categories, with a focus on comparing GED holders to HS graduates. Logistic regression was used to predict the odds of current cigarette smoking and successful quitting, accounting for demographic, employment, family/sociocultural, mental health, and other potential confounders. RESULTS: The smoking rate among adults with a GED (44.1%) was more than five times the rate for those with a college degree (8.3%) and almost twice the rate of adults whose highest level of education was a high school diploma (23.6%). GED holders were also more likely to have started smoking before the age of 15 (32.2%) compared with HS graduates (12.2%) (p<0.001). Even after controlling for 23 socio-demographic and health characteristics, GED holders retained significantly higher odds of current smoking compared to HS graduates (OR=1.73; 95% CI: 1.56, 1.93) and significantly lower odds of successful quitting (OR=0.83, 95% CI: 0.73, 0.94). CONCLUSIONS: GED holders had greater odds of being a current cigarette smoker, regardless of other characteristics that usually explain smoking. Earlier smoking initiation among GED holders, in combination with lower odds of quitting, contributed to their higher current smoking rate. |
Mortality risks associated with average drinking level and episodic heavy drinking
Schoenborn CA , Stommel M , Ward BW . Subst Use Misuse 2014 49 (10) 1250-8 Data from the 1997 to 2004 National Health Interview Survey Sample Adult questionnaires were linked to the National Death Index (N = 242,397) to examine mortality risks associated with average and episodic heavy drinking. Cox proportional hazard models (Stata 12.0) revealed that (average) heavier drinkers and episodic heavy drinkers (5+ in a day) had increased mortality risks but when examined together, episodic heavy drinking added only modestly to the mortality risks of light and moderate drinkers. Limitations and implications of results for survey measurement of potentially harmful levels of alcohol use are noted. This was a Federal study that received no outside funding. |
Adherence to the 2008 adult physical activity guidelines and mortality risk
Schoenborn CA , Stommel M . Am J Prev Med 2011 40 (5) 514-21 BACKGROUND: Mortality differentials by level and intensity of physical activity have been widely documented. A comprehensive review of scientific evidence of the health benefits of physical activity led the USDHHS to issue new Federal Guidelines for physical activity in 2008. Reductions in mortality risk associated with adherence to these Guidelines among the general U.S. adult population have not yet been studied. PURPOSE: This study compared the relative mortality risks of U.S. adults who met the 2008 Guidelines with adults who did not meet the recommendations. METHODS: Cox proportional hazards models were used to examine the relative mortality risks of U.S. adults aged ≥18 years, using data from the 1997-2004 National Health Interview Survey and linked mortality records for deaths occurring in 1997-2006 (analyzed in 2010). Risks for adults with and without chronic health conditions were examined separately. RESULTS: Meeting the recommendations for aerobic activity was associated with substantial survival benefits, especially among the population having chronic conditions, with estimated hazard ratios ranging from 0.65 to 0.75 (p<0.05). While strengthening activities by themselves did not appear to reduce mortality risks, they may provide added survival benefits to those already engaged in aerobic activities. The relative benefits of physical activity were greatest among adults who had at least one chronic condition. CONCLUSIONS: Adherence to the 2008 Physical Activity Guidelines was associated with reduced all-cause mortality risks among U.S. adults, after controlling for sociodemographic characteristics, BMI, smoking, and alcohol use. |
Variations in BMI and prevalence of health risks in diverse racial and ethnic populations
Stommel M , Schoenborn CA . Obesity (Silver Spring) 2010 18 (9) 1821-6 When examining health risks associated with the BMI, investigators often rely on the customary BMI thresholds of the 1995 World Health Organization report. However, within-interval variations in morbidity and mortality can be substantial, and the thresholds do not necessarily correspond to identifiable risk increases. Comparing the prevalence of hypertension, diabetes, coronary heart disease (CHD), asthma, and arthritis among non-Hispanic whites, blacks, East Asians and Hispanics, we examine differences in the BMI-health-risk relationships for small BMI increments. The analysis is based on 11 years of data of the National Health Interview Survey (NHIS), with a sample size of 337,375 for the combined 1997-2007 Sample Adult. The analysis uses multivariate logistic regression models, employing a nonparametric approach to modeling the BMI-health-risk relationship, while relying on narrowly defined BMI categories. Rising BMI levels are associated with higher levels of chronic disease burdens in four major racial and ethnic groups, even after adjusting for many socio-demographic characteristics and three important health-related behaviors (smoking, physical activity, alcohol consumption). For all population groups, except East Asians, a modestly higher disease risk was noted for persons with a BMI <20 compared with persons with BMI in the range of 20-21. Using five chronic conditions as risk criteria, a categorization of the BMI into normal weight, overweight, or obesity appears arbitrary. Although the prevalence of disease risks differs among racial and ethnic groups regardless of BMI levels, the evidence presented here does not support the notion that the BMI-health-risk profile of East Asians and others warrants race-specific BMI cutoff points. |
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